Chip-based genotyping by mass spectrometry
收藏PubMed Central1999-08-31 更新2026-04-25 收录
下载链接：
https://pmc.ncbi.nlm.nih.gov/articles/PMC17834/
下载链接
链接失效反馈官方服务：
资源简介：
Silicon chips with immobilized target DNAs were used for accurate genotyping by mass spectrometry. Genomic DNAs were amplified with PCR, and the amplified products were covalently attached to chip wells via N-succinimidyl (4-iodoacetyl)aminobenzoate (SIAB) chemistry. Primer annealing, extension, and termination were performed on a 1-μl scale directly in the chip wells in parallel. Diagnostic products thus generated were detected in situ by using matrix-assisted laser desorption ionization mass spectrometry. This miniaturized method has the potential for accurate, high-throughput, low-cost identification of genetic variations.
提供机构：
National Academy of Sciences
创建时间：
1999-08-31
