Ultra-Deep Targeted Sequencing

Ultra-deep targeted sequencing (UDT-Seq) can identify subclonal somatic mutations in tumor samples. Early assays limited breadth and depth restrict their clinical utility. Here, we target 71kb of mutational hotspots in 42 cancer genes. We present novel methods enhancing both laboratory workflow and mutation detection. We evaluate UDT-Seq true sensitivity and specificity (>94% and >99%, respectively) for low prevalence mutations in a mixing experiment and demonstrate its utility using 6 tumor samples. With an improved performance when run on the Illumina Miseq, the UDT-Seq assay is well suited for clinical applications to guide therapy and study clonal selection in heterogeneous samples.