Culture media strongly influences primary human bronchial epithelial cells' transcriptomic response to ozone exposure

Exposure to the ambient air pollutant ozone induces acute and chronic respiratory health effects in part by causing inflammation of the airways. Several aspects of the inflammatory response to ozone can be modeled in vitro using primary human bronchial epithelial cells (HBECs) cultured at an air-liquid interface. We tested two commonly used HBEC culture media systems, one proprietary and one non-proprietary, to identify which system yielded the most in vivo-like pro-inflammatory response to acute ozone exposure as indicated by gene expression. Cells from six donors were grown in each culture system in parallel followed by examination of epithelial morphology and cell type proportions prior to ozone exposure. Cultures grown in the proprietary system were notably thicker and contained more ciliated and secretory cells, as well as internal cyst-like structures. The transcriptomic response to acute ozone exposure (0.5 parts per million ozone x 2 hours) was strongly affected by media. HBECs ..., , # Culture media strongly influences primary human bronchial epithelial cells' transcriptomic response to ozone exposure

Dataset DOI: [10.5061/dryad.qfttdz0t4](10.5061/dryad.qfttdz0t4)

## Description of the data and file structure

Transcriptomic data for differential expression and gene set enrichment analysis to compare primary bronchial epithelial cell responses to ozone exposure when grown in different culture medias.

### Files and variables

#### File: TableS1_UNC_O3vsFA.xlsx

**Description:** Full DESeq2 results from ozone vs filtered air-exposed UNC-grown cultures. Missing values indicated with blank cells.

##### Variables

* \"hgnc_symbol\" = HGNC approved symbol for the accompanying Ensembl ID.
* \"baseMean\" = Mean base expression level across all samples after DESeq2 library size normalization.
* \"log2FoldChange\" = log2 fold change calculated by DESeq2 for the given comparison.
* \"lfcSE\" = standard error of the log2 fold change values.
* \"pvalue\" = nominal p value calculated b...,