Summary of parameters and results for M-ratio analysis and BOTTLENECK 1.2 software used to detect significant reduction in effective population sizes.

N—sample size; θ = 4Neμ; Ne—pre-bottleneck effective population size, corresponding to given value of θ and mutation rate μ = 10–5; Simulation results—percentage of times when smaller M-ratio at equilibrium is expected, MC—critical value of M (95% of equilibrium values of M should be above MC), Heterozygote excess—P-value of Wilcoxon sign-rank test for heterozygote excess relative to population in mutation-drift equilibrium).