Dataset of SARS CoV-2 in wastewater in Örebro, Sweden

This is a metadata record for a continuously updated dataset of SARS-CoV-2 RNA data in wastewater in Örebro, Sweden.The dataset is part of a research study led by associate professor Maja Malmberg (SLU, Swedish University of Agricultural Sciences) and her research group in collaboration with associate professor Mette Myrmel at the Norwegian University of Life Sciences. The amount of SARS-CoV-2 virus is measured in wastewater from the wastewater treatment facility in Örebro. The research group is part of the Environmental Virus Profiling Research Area of the SciLifeLab National COVID-19 Research Program.https://www.scilifelab.se/covid-19/national-program/environment/After preparation, the viruses are extracted using ultra filtration and analyzed using qPCR technique for SARS CoV-2 RNA. RT-qPCR samples are normalized against Pepper Mild Mottle Virus (PMMoV). From Oct 2020 until January 2021, three samples per week were taken and an average of the three values was calculated to provide a weekly estimate. From February to March 2021, samples were only collected at one time point per week, and from April onwards two samples per week are analysed and the average presented. Primers used to detect the SARS-COV-2 gene have been previously used and verified by Corman and colleagues, 2020.For more information see https://www.covid19dataportal.se/data_types/environment/wastewater/ The dataset started week 44 2020 and updates every second week.The dataset is available as part of the Environmental Virus Profiling data section "The amount of SARS-CoV-2 virus in wastewater across Sweden" https://www.covid19dataportal.se/data_types/environment/wastewater/#orebroon the Swedish COVID-19 Data Portal (https://covid19dataportal.se ).Funded by the SciLifeLab/KAW National Covid-19 Research Program•https://www.scilifelab.se/covid-19/national-program

本记录为瑞典厄勒布鲁市持续更新的SARS-CoV-2 RNA废水数据集的元数据。该数据集由瑞典农业大学（SLU）副教授Maja Malmberg及其研究团队领衔，与挪威生命科学大学副教授Mette Myrmel合作开展的研究项目所组成。研究团队隶属于SciLifeLab国家COVID-19研究计划的环境病毒分析研究领域。SARS-CoV-2病毒含量通过分析厄勒布鲁废水处理厂的水样进行测定。病毒提取采用超滤技术，RNA分析则采用实时定量PCR（qPCR）技术。RT-qPCR样本以辣椒花叶病毒（PMMoV）为参照进行标准化。自2020年10月至2021年1月，每周采集三个样本，并对三个值进行平均计算，以提供每周的估计值。2021年2月至3月，每周仅采集一次样本，自4月起每周分析两个样本，并呈现平均值。用于检测SARS-CoV-2基因的引物已由Corman等人在2020年使用并验证。更多详细信息请见https://www.covid19dataportal.se/data_types/environment/wastewater/。该数据集自2020年第44周开始，每两周更新一次。数据集作为环境病毒分析数据部分的“瑞典废水中的SARS-CoV-2病毒含量”可用（https://www.covid19dataportal.se/data_types/environment/wastewater/#orebro）。该数据集可在瑞典COVID-19数据门户（https://covid19dataportal.se）的“环境病毒分析”数据部分获取。研究资金由SciLifeLab/KAW国家COVID-19研究计划提供。更多信息请访问https://www.scilifelab.se/covid-19/national-program/。