A pulmonary vascular model from endothelialized whole organ scaffolds

We introduce a novel in vitro platform that could simulate key native pulmonary vascular phenotypes and functions, and shows the potential of using the bioengineered whole organ as a platform for disease modeling. Since it is becoming clear that vascular endothelium and the coagulation cascade are adversely affected in some COVID19 patients, such an engineered organ system may play an important role in dissecting disease mechanisms and treatments. Overall design: Single-cell RNA sequencing data was generated from native pulmonary endothelium, primary pulmonary endothelium cultured on tissue flasks, primary pulmonary endothelium cultured in acellular lung scaffolds, induced pluripotent stem cell-derived endothelium cultured on tissue flasks, and induced pluripotent stem cell-derived endothelium cultured in acellular lung scaffolds