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Gene expression profile of B cells from OPN-competent and deficient Faslpr autoimmune mice

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE193790
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We performed gene expression profile on splenic CD19+ cells from autoimmune Faslpr/lpr and OPN-/-Faslpr/lpr mice to investigate the dynamics at the basis of the more incident transformation characterizing OPN-deficient animals. GEP on splenic CD19+ from BALB/c and OPN-/- mice was performed as control. CD19+ cells were separated through immunomagnetic beads (anti-CD19) from the splenic cell suspension of 5 month-Faslpr/lpr and OPN-/-Faslpr/lpr mice. CD19+ from BALB/c and OPN-/- animals were analysed as controls. After check of purity, RNA was extracted and GEP was performed.
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2022-12-22
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