Gene expression profile of B cells from OPN-competent and deficient Faslpr autoimmune mice

We performed gene expression profile on splenic CD19+ cells from autoimmune Faslpr/lpr and OPN-/-Faslpr/lpr mice to investigate the dynamics at the basis of the more incident transformation characterizing OPN-deficient animals. GEP on splenic CD19+ from BALB/c and OPN-/- mice was performed as control. CD19+ cells were separated through immunomagnetic beads (anti-CD19) from the splenic cell suspension of 5 month-Faslpr/lpr and OPN-/-Faslpr/lpr mice. CD19+ from BALB/c and OPN-/- animals were analysed as controls. After check of purity, RNA was extracted and GEP was performed.