Identification of exosomal miRNAs specifically derived from intrafollicular cells involved in polycystic ovary syndrome

Exosomes have recently been shown to play a key role in cell-to-cell communication through delivery of various functional content, including microRNAs (miRNAs). We investigated the potential roles of exosomal miRNA derived intrafollicular cells in polycystic ovary syndrome (PCOS). Using microarray profiling, a total of 492 miRNAs and 220 miRNAs were found in follicular fluid-derived exosomes and serum-derived exosomes, respectively, in PCOS and non-PCOS females. By excluding miRNAs existing in serum-derived exosomes, we found 179 miRNAs which were specifically expressed in follicular fluid-derived exosomes both in PCOS and non-PCOS females. Using microarray profiling, a total of 492 miRNAs and 220 miRNAs were found in follicular fluid-derived exosomes and serum-derived exosomes, respectively, in PCOS and non-PCOS females. By excluding miRNAs existing in serum-derived exosomes, we found 179 miRNAs which were specifically expressed in follicular fluid-derived exosomes both in PCOS and non-PCOS females. Exosomes were isolated from pooled follicular fluid of PCOS and non-PCOS females, respectively. Serum-derived exosomes were isolated from pooled serum of PCOS and non-PCOS females, respectively. miRNA profiles were generated using microarray. Comparison of miRNAs expression in fluid-derived exosomes and serum-derived exosomes were preformed to identify the exosomal miRNAs specifically derived from intrafollicular cells