An effective protocol for analyzing the Anxiolytic Effects of bioactive oligosaccharide and Reverse the Impact of Chronic physical Stress in Mice based on Gut microbiota modulation

· <b>In vivo evaluation - Antistress efficacy in mice Model</b> Animals and maintenance protocol Physical stress induction in mice Weight and feed intake measurement· <b>Behaviour analysis based on stress induction</b> Elevated plus-maze test Open-field test Novel object recognition test· <b>Neurotransmitter </b>- Corticosterone and serotonin Levels determination· <b>Screening of microbiota modulation based on metagenomics analysis</b>Next-generation sequence (NGS) - Illumina sequence workflowNGS data analysisQuality assessment and sample sequencingMetagenomics Data Analysis Data filtering and Normalization<br>