Metatranscriptomes: Gulf of Mexico Open Ocean (GOMOO) and Gulf of Mexico Coastal Waters (GOMCOAST)

Mesocosm experiments were carried out to test the effect of oil and Corexit on microbial community gene expression over time. Four mesocosm treatments were prepared in triplicate for this study: (1) Control (seawater only); (2) Water accommodated fraction (WAF; oil + seawater); (3) Chemically-enhanced WAF (CEWAF; oil + Corexit + seawater); (4) Diluted CEWAF (DCEWAF; 1:10 dilution). Water was collected from each mesocosm and filtered (47mm, 0.2um PES membranes). Total RNA was extracted from filtered water samples and sequenced using Illumina HiSeq 2500.