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Raw data for whole plasmid and whole genome sequencing

NIAID Data Ecosystem2026-05-02 收录

微生物基因工程

CRISPR基因调控

数据链接：

https://zenodo.org/record/13370375 数据链接链接失效反馈

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资源简介：

Original data for plasmid and genomic DNA sequencing in the paper: Tailoring Microbial Fitness Through Computational Steering and CRISPRi-Driven Robustness Regulation

应用场景：

创建时间：

2024-11-01

CRISPR转座子

微生物基因工程

Genomic integrations in E. coli (DH10B) using a system robustly integrates a linear DNA payload by employing a Type I-F CRISPR-associated transposon from Vibrio cholerae.

NIAID Data Ecosystem100

Scaffold RNA engineering in type V CRISPR-Cas systems: a potent way to enhance gene expression in the yeast Saccharomyces cerevisiae.

CRISPR基因调控

酵母合成生物学

New, orthogonal transcription factors in eukaryotic cells have been realized by engineering nuclease-deficient CRISPR-associated proteins and/or their guide RNAs. In this work, we present a new kind o

Alliance of Genome Resources30

CRISPRi RNA-seq from K562 (ENCSR243VNX)

CRISPR基因调控

HMGN2功能研究

RNA-seq on K562 cells treated by CRISPR interference targeting HMGN2. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCO

NIAID Data Ecosystem10

Escherichia coli BW25113 JME78 resequencing genome sequencing. Escherichia coli strain:BW25113 JME78

微生物基因工程

木质素降解

Resequencing of E. coli BW25113 mutant strain modified to increase its ability to degrade lignin compounds and to tolerate the harsh conditions of lignocellulosic fermentations

NIAID Data Ecosystem60

MYC and GATA1 CRISPRi validation sgRNAs.

CRISPR基因调控

sgRNA验证

Guide sequences are given for each target. The positive control targets a known functional sequence (e2) identified by Fulco et al. As negative controls a dCas9:KRAB only or an sgRNA targeting a non-f

NIAID Data Ecosystem30

Raw data for whole plasmid and whole genome sequencing

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