mRNA profiles of active hematopoieitc stem cells

Purpose: The goals of this study are to elucidate the underlying mechanism for the activation of HSCs Methods: After wild type (WT) HSCs (CD150+ CD48- EPCR+ Lineage-) were treated with 5-fluorouracil (5-FU), 100 cells of HSCs were sorted. Moreover, after the culture with or without Nifedipien, cultured HSC fraction (CD150+ CD48- EPCR+ c-kit+ Sca-1+ Lineage-) were sorted. These cells were subjected to mRNA sequence using Next-seq (n>3). The sequence reads that passed quality filters were analyzed by CLC genomic workbench. After the culture with or without Nifedipine, HSCs wihtin 3 cell division population were sorted, and subsequently mRNA profiles were generated by deep sequencing using Next-seq (n>3). Using HSCs derived from untreated and 5-FU-treated mice, mRNA profiles were generated by deep sequencing using Next-seq (n>4).