RNAseq profiling of Col-0 and ex1ex2 mutant inoculated by fungus Alternaria alternata in Arabidopsis

The signaling function of singlet oxygen (1O2) has been recognized only recently in plants. Nevertheless, response mechanisms of 1O2 signaling under different stress conditions still remain unknown. To discover the specific contribution of 1O2-mediated and EXECUTER(EX)-dependent signaling during disease development of Arabidopsis, RNAseq was conducted to analyse the expression pattern of 1O2 response genes in Col-0 and ex1ex2 mutant plants after inoculation of necrotrophic Alternaria alternata wild type or TeA toxin-deficient mutant DHP001 strains. Methods: RNAseq libraries were sequenced on an Illumina Hiseq X Ten plantform with standard Illumina protocols. Results: A. alternata upregulated significantly the expression level of 1O2 response genes, inactivation of EX suppressed their expression. Overall design: at least three 3-wk-old Col-0 or ex1ex2 mutant plants, inoculated with 3-mm-PDA plugs containing A. alternata actively mycelia or 3-mm-PDA plugs without mycelia in vivo. After 3, 6 and 12 h treatment, two rosette leaves per plant were sampled and stored immediately in liquid nitrogen. Three independent experiments were done. Data were analysed on the basis of treated vs untreated comparison.