Overexpression of RNA deaminase ADAR2 in Th17 cells

ADAR2 is induced upon T cell activation and its immunological function is not known. Here, we performed RNA-seq on control and Th17 cells overexpressing wildtype or catalytic dead ADAR2 to identify A-to-I edited transcripts that are targets of ADAR2. Cultured cells from two independent wildtype mice were used. Results of ADAR2 overexpressed (ADAR2_WT) or enzymatic dead (ADAR2_E396A) Th17 cells were compared to Th17 cells from the same donor transduced with the MSCV empty vector.