Additional file 9 of RNA-seq: technical variability and sampling

Additional file 9:Agreement between technical replicates and biological replicates for RPKM measured on common contigs. A comparison in the agreement of the estimation of the amount of expression. Agreement in whether common contigs are expressed, expressed at a low level (0 < RPKM < 20), and expressed at a high level (RPKM > 20) and agreement on a 9 level ordinal scale as follows: RPKM less than 10, 20, 40, 80, 160, 320, 1000 and greater than 1000. Agreement was measured using a kappa coefficient. The number of common contigs where disagreement is greater than 2 logs and greater than one log are also given. Format XLS. View with Excel. (XLSX 10 KB)