Mammalian core histones were identified as DEN2 C binding partners.
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The 100 amino acid mature DEN2 C protein fused to streptavidin binding protein and calmodulin binding protein (CBP) tags was expressed in Huh7 liver cells and interactions identified using a tandem affinity purification assay. Proteins that bound tagged DEN2 C were isolated and identified by LC-MS/MS spectrometry.
创建时间：
2011-09-01
