Correlation between rCRT oligomerization and immunological activity.

a)Relative macrophage stimulation activity (RMSA) of CRT is estimated using nCRT as reference, which is able to induce TNF-α production by macrophages in vitro at a concentration of 10 µg/ml or above (see Fig. 5A). The listed results are based on several batches of independent experiments including Fig. 5A.b)Ability to elicit specific IgG responses in healthy BALB/c mice after s.c. immunization, in the absence of adjuvant, with 100 µg protein and a booster immunization with 50 µg protein a fortnight later. The mice were monitored for up to 28 days after the second immunization. ND: not detected; -: not immunogenic; +: strong humoral response; −/+: weak response.c)As determined by Native-PAGE.