Data underlying the research of: The mRNA expression of CACYBP in bladder and kidney cancer.
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<strong>qRT-PCR </strong> Cellular RNA was extracted using Trizol, and total RNA (500 ng) was transcribed into cDNA using the PrimeScript kit. Actin primers were used as an internal control. Real-time fluorescence quantitative RT-PCR assays were performed using Lightcycler 480ii. The qRT-PCR primer sequences used in this study are shown below: Cacybp: forward primer: 5-CTCCCATTACAACGGGCTATAC-3, reverse primer: 5-GAACTGCCTTCCACAGAGATG-3; hactin: forward primer: 5-GGCATCGTCACCAACTGGGAC-3; reverse primer: 5-CGATTTCCCGCTCGGCCGTGG-3. Obtain the Cq datas of the actin and CACYBP gene of HK-2, O-786, SV-HUC-1, T24 cells for analysis.
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4TU.ResearchData
创建时间:
2023-02-08



