Detection of Eepd1 myristoylation in vivo by data-independent acquisition mass spectrometry (DIA-MS)

Eight 8-week-old male C57BL/6J mice were injected with adenovirus expressing Eepd1-Flag (3×10^8/mouse) into multiple sites in their iWAT. Three days later, each mouse was administered 50 mg/kg of myristic acid solution (prepared in 0.1% BSA) via oral gavage. Half of the mice were then exposed to 8°C for 16 hours in a cold chamber, while the other half remained at room temperature (4 mice per group).