Runx3 function in CD4+ splenic dendritic cells

CD4+ dendritic cells are part of the innate immunity essential for priming and activating of CD4+ T cells To identify Runx3 responsive genes CD4+ dendritic cells were sorted from freshly isolated macs enriched splenic DCs taken from 6 weeks old mice. Six samples from six mice were sorted and analyzed where in each littermates pair consisted of a control and Runx3 KO.CD4阳性树突状细胞（CD4+ dendritic cells）属于固有免疫组分，对CD4阳性T细胞的启动与激活过程不可或缺。为鉴定Runx3响应基因，研究人员从6周龄小鼠的新鲜分离、经磁激活细胞分选（MACS）富集的脾脏树突状细胞（splenic DCs）中分选得到CD4阳性树突状细胞。本次实验共对6只小鼠的6份样本完成分选与分析，每一组同窝仔鼠对均包含1只对照小鼠与1只Runx3基因敲除（Runx3 KO）小鼠。