Is axonal iron transport in neurons mediated by the lysosomes?

It is unclear why and how iron accumulates in certain brain regions implicated in neurodegenerative disorders, as well as how iron can move from one brain region to another. We suggest that the axonal lysosomal pathway could transport iron in the brain. The main objective of this project is to detect the presence of iron in the lysosomes along the axons of primary rat hippocampal neurons. We will also investigate the effect of iron overload on iron re-distribution in axons and neurons. These objectives can only be demonstrated using a correlative approach combining high spatial resolution and high sensitivity SXRF (Synchrotron X-ray Fluorescence) iron imaging with super-resolution microscopy of lysosomes in axons. This research will serve as a fundamental basis for future studies to develop new treatments for neurodegenerative disorders targeting lysosomal iron.