Involvement of Arabidopsis TUTases in viral RNA uridylation
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE212293
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资源简介:
Using 3' RACE-seq method, we analyzed the impact of URT1 and/or HESO1 inactivation on the uridylation of GFLV, TuMV and TCV RNAs in Arabidopsis. 3’ RACE-seq method was used to measure poly(A) tail sizes and analyze uridylation levels. This protocol is based on the ligation of a primer at the 3’ end of RNA, and the subsequent targeted amplification by PCR of amplicons suitable for Illumina sequencing.
创建时间:
2023-04-27



