Effect of column cleanup on T7 based RNA linear amplification
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Effect of column cleanup on T7 based RNA linear amplification
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2005-11-03
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Comparison of linear and exponential amplification techniques for expression profiling at the single-cell level
We tested the performance of three methods for amplifying single-cell amounts of RNA under ideal conditions: T7-based in vitro transcription; switching mechanism at 5' end of RNA template (SMART) PCR
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Additional file 4: of Assessing characteristics of RNA amplification methods for single cell RNA sequencing
Number of detected genes for low-depth in silico samples. As Fig. 2a, except that each dilution replicate has been subsampled to a depth of 500,000 unique genic reads. (XLS 74 kb)
Figshare2016-12-14 更新40
Additional file 1: of Assessing characteristics of RNA amplification methods for single cell RNA sequencing
Control dataset sample identification, protocol information, and RNA sequencing stats. Experimental group, protocol information and RNA sequencing statistics for each sample used in primary analyses.
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Effect of the amount of input total RNA on T7 amplification
Zhao et al. Amplification Table 9 This experiment was designed to evaluate the effect of the amount of input total RNA on the fidelity, reproducibility, and yield of T7 based RNA linear amplification.
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Additional file 5: of Assessing characteristics of RNA amplification methods for single cell RNA sequencing
Gene detection logistic regression model. See model details in Methods . Abbreviations: M expected number of input molecules; L gene length (kilobases); G gene GC content; S strength of gene local sec
Figshare2016-12-14 更新30
