Table 1 in Nudibranch molluscs of Sakhalin Island, Northwestern Pacific: new records and descriptions of two new species

Table 1. Amplification and sequencing primers and PCR conditions. Табл. 1. Праймеры, испольЗованные длЯ амплификации и секвенированиЯ, и условиЯ ПЦР.
MarkerPrimersPCR conditionsReferenceLCO1490 GGT CAA CAA ATC ATA AAG ATA TTG G HCO2198 TAA ACT TCA GGG TGA CCA AAA AAT CA jgLCO5 min - 95°C, 35x[15 s - 95°C, 45 s - 45°C, 1 min - 72°C], 7 min - 72°CFolmer et al. 1994Cytochrome c oxidase subunit ITNT CNA CNA AYC AYA ARG AYA TTG G jgHCO ANA CYT CNG GRT GNC CRA ARA AYC A polyLCO GAY TAT WTT CAA CAA ATC ATA AAG ATA TTG G polyHCO TAM ACT TCW GGG TGA CCA AAR AAT CA5 min - 95°C, 10x[30 s – 95°C, 1 min - 52°C (-0.5°C/cycle), 1 min - 72°C], 30x [30 s – 95°C, 1 min – 48°C, 1 min - 72°C], 7 min - 72°CGeller et al., 2013 Carr et al., 201116S rRNA16Sar-L CGC CTG TTT ATC AAA AAC AT 16S R CCG RTY TGA ACT CAG CTC ACG5 min - 95°C, 35x[20 s - 95°C, 30 s - 52°C, 45 s - 72°C], 7 min - 72°CPalumbi et al. 1991 Puslednik and Serb 2008Histone H3H3AF ATG GCT CGT ACC AAG CAG ACV GC H3AR ATA TCC TTR GGC ATR ATR GTG AC5 min - 95°C, 35x[15 s - 94°C, 30 s - 50°C, 45 s - 72°C], 7 min - 72°CColgan et al., 1998SequencingM13F TGT AAA ACG ACG GCC AGT M13R CAG GAA ACA GCT ATG ACTn/an/an/an/a