Fitness of 14 TDH3 promoter variants in YPD

Determining the fitness consequences of changes in the expression level of the yeast gene TDH3.
Conclusion:
The relationship between TDH3 expression level and fitness in YPD is non-linear and presents a plateau of maximal fitness located around the wild type level of expression.
Notes:
Fitness was quantified by competition between each tested strain expressing YFP against a common competitor expressing GFP. The relative frequency of YFP and GFP cells present in each population was recorded at four time points. The FCS data were collected from seven plates of 96 samples scored at four different time points and in 3 separate experiments including one or three plates each. Each plate contains 24 replicates of the control YFP strain with a wild type copy of TDH3 competed against the GFP competitor. These controls can be used to correct for variation existing between plates.