New approaches to the production of certified varieties of apple trees using NGS diagnostics of pathogens

Implementation and evaluation of tests (PCR, ELISA, NGS) for the presence of viruses, viroids and phytoplasma in propagation materials of apple. Research and application of new sanitation techniques using promising antiviral substances, or cryo-knife. Confirmation of health status of sanitated cultivars including use of modern diagnostic methods such as high-throughput sequencing (NGS). Overall design: 16 sRNA libraries were prepared and used to survey viromes of apple trees - no replicate samples were prepared