Dataset_Spore Cryopreservation and Sporeling growth

Spores of Isoetes cangae were obtained from specimens collected in situ. Samples were submitted to three different moisture conditions: hydrated, air-dry desiccation, and drying in silica gel. Desiccated samples were stored in an ultrafreezer (-80°C) or liquid nitrogen (-196°C) and the hydrated spores at room temperature. The spore viability was evaluated through spore germination (G) and sporeling emergence (SE) indices after 30, 90, 180, and 275 days of storage. The growth of emerged sporophytes was evaluated after two months.