Transcriptome comparison of Col-0, se-1, ago1-27 and se-1 35S::MIR156 in Arabidopsis thaliana.. Arabidopsis thaliana

Microarray analysis of wild type plants and plants with reduced (ago1-27 and se-1) or increased miR156 levels (se-1 p35S:MIR156). Shoot apices were dissected from 20-day-old, short-day grown plants. Overall design: Plants are grown in short day for 20 days. Total RNAs from shoot apex are extracted by RNeasy Plant Mini kit (Qiagen). The probes are labeled with MessageAmp-Biotin Enhanced, single round aRNA amplification kit from Ambion and hybridized to Affy ATH1 array. Each genotype has two replicates.