10x single cell sequencing for old mouse transcriptomes after sevoflurane exposure

Although previous studies have confirmed that sevoflurane treatment has an effect on neurological function of old mouse, the underlying molecular mechanism remain unclear. In the present study, we performed scRNA-Sequencing (scRNA-Seq) analysis in the hippocampus from brain of old mouse . We thus isolated hippocampus from brain of old mouse, with or without sevoflurane exposure, and performed single cell sequencing. Single cell sequencing is a innovative experimental procedures which allows the identification of various subsets of cells in the neverous system after sevoflurane exposure. Overall design: Six elderly mice were treated with sevoflurane or air.2% concentration for 6 hours. The mouse hippocampus was taken 24 h later for single-cell sequencing