Raw data supporting: D-Enantiomeric β-Casein 197 Disrupts Biofilm-Associated Infection of MDR Pseudomonas aeruginosa and Acinetobacter baumannii through Superior LPS Binding and Host Cell Protection

This dataset contains all raw data supporting the findings reported in the manuscript: "D-Enantiomeric β-Casein 197 Disrupts Biofilm-Associated Infection of MDR Pseudomonas aeruginosa and Acinetobacter baumannii through Superior LPS Binding and Host Cell Protection."File descriptions:Biofilm_RawData.xlsx — Raw OD570 values from crystal violet biofilm inhibition and eradication assays against four MDR P. aeruginosa (R1-R4) and four MDR A. baumannii (13-16) clinical isolates (n=5 per group).Cell viability_RawData.xlsx — Raw CCK-8 assay OD450 values for cytotoxicity evaluation of L- and D-β-casein 197 against A549 and J774A.1 cells at concentrations of 12.5, 25, 50, and 100 μM (n=3).FlowCyto_RawData.xlsx — Quadrant population percentages (Q1-Q4) from SYTO9/PI flow cytometry membrane integrity assays against all tested MDR isolates (n=3 per group).Flow_Cytometry_Raw_Plots.pdf — Representative raw dot plots from flow cytometry analysis showing gating strategy and quadrant populations.Galleria_RawData_Figshare.xlsx — Individual larval survival data from Galleria mellonella infection model (n=15 per group, 96 h observation, MDR P. aeruginosa R1 and A. baumannii 13).ITC_RawData.xlsx — Raw isothermal titration calorimetry data (heat per injection, normalized heat, molar ratio) from three independent replicates of L- and D-β-casein 197 binding to LPS.LDH_CFU_RawData.xlsx — Raw OD490 values from LDH release assay and CFU counts from bacterial internalization assay in J774A.1 macrophages infected with MDR P. aeruginosa and A. baumannii (n=3 per group).TEM — Transmission electron microscopy original images of untreated, L-form-treated, and D-form-treated MDR P. aeruginosa and A. baumannii cells.Biofilm CLSM.zip — Original confocal laser scanning microscopy images from biofilm inhibition and eradication assays stained with SYTO9/PI.All data are released under CC BY 4.0 licence.