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Lentiviral vector choice for CRISPR delivery affects the false-positive rate of genome-wide CRISPR screens

NIAID Data Ecosystem2026-03-14 收录

CRISPRi筛选

慢病毒载体

数据链接：

https://www.ncbi.nlm.nih.gov/bioproject/PRJNA893023 数据链接链接失效反馈

官方服务：

资源简介：

Lentivirus construct comparison for CRISPR screen

应用场景：

创建时间：

2022-10-21

相关数据集

raw data for main figures.xlsx

CRISPRi筛选

功能基因组学

data used to create main figures for: Park et. al., Efficient prioritization of CRISPR screen hits by accounting for targeting efficiency of guide RNA, BMC Biol, 2023.

Figshare2023-01-28 更新70

Essential gene profiles for human pluripotent stem cells identify uncharacterized genes and substrate dependencies. Mair et al.

干细胞鉴定

CRISPRi筛选

Human pluripotent stem cells (hPSCs) provide an invaluable tool for modeling diseases and hold promise for regenerative medicine. For understanding pluripotency and lineage differentiation mechanisms,

NIAID Data Ecosystem20

Sequences of primers used for lentivirus construction.

慢病毒载体

引物设计

Sequences of primers used for lentivirus construction.

Figshare2015-12-02 更新10

Bcor loss perturbs myeloid differentiation and promotes leukaemogenesis [TargetedCRISPRscreen]

白血病发病机制

CRISPRi筛选

Investigating Bcor target genes in BcorΔE9-10KrasG12D leukaemia with a custom CRISPR dropout screen BcorΔE9-10KrasG12D cell line was transduced with a custom CRISPR library and a dropout screen was pe

NIAID Data Ecosystem30

Genes of CRISPR-Cas9 negative screening.

CRISPRi筛选

肿瘤免疫逃逸

We compared changes in sgRNA abundance between T24 cells challenged and unchallenged by CD8+ T cells, and then performed negative screening (p < 0.01, log2Fold change < −1.0, and good sgRNAs ≥ 5). (X

NIAID Data Ecosystem10