scRNA-seq data of peritoneal immune cells from PTENα knockout and WT mice

To determine the changes in peritoneal immune cells upon PTENα deficiency, we performed scRNA-seq to analyze the peritoneal immune microenvironment in wild-type (WT) and PTENα knockout (KO) mice. Wild-type (WT) and PTENα knockout (KO) mice were placed on a high-fat diet beginning at 4 weeks of age and maintained for three months. To obtain peritoneal immune cells, mice were intraperitoneally injected with serum-free RPMI 1640 medium, and the lavage fluid was collected. CD45⁺ immune cells were then isolated by flow cytometry and subjected to single-cell RNA sequencing to profile the peritoneal immune microenvironment.