Integrated analysis of long non-coding RNAs expression profiles in THP-1 induced M0, M1 and M2 macrophage and GCC culture medium induced similar M2 (CM-sM2)

We used microarray and bioinformatics analysis to find potential lncRNAs which may be useful in the polarization of macrophages To screen for the functional lncRNAs involved in macrophages polarization, total RNA from THP-1 induced M0, M1, M2 and CM-sM2 cells were extracted and analyzed, we employed SBC-lncRNA (human 4*180K) microarray as a discovery platform to identify genes. [M0 were induced by PMA (50ng/ml) for 48h, M1 were induced by LPS (100ng/ml) and IFN-γ (20ng/ml) for 48h and M2 were induced by IL-4 (20ng/ml) for 48h both on the basis of M0. CM-sM2 were induced by 30% culture medium of gastric cancer cells on the basis of M0.]