BlueDevil
收藏DataONE2014-04-15 更新2024-06-27 收录
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资源简介：
A Python scripts to extract gene homologs from 1KP transcriptomes. Sequences for gene-of-interest are queried by tBLASTn and the significant hits to transcriptome scaffolds are extracted. For each scaffold, the best open reading frame is identified, and the sequence is translated into amino acids and then BLASTp queried against the NCBI non-redundant protein database (nr). The scaffolds is discarded if they did not match the homologs in the nr database with an e-value threshold of <0.001. The filtered scaffolds from SOAP de novo and SOAP de novo trans assemblies are then merged using CAP3.
创建时间：
2014-04-15
