Supplemental Material for Nageshan, Krogan, and Cooper, 2024

<strong>Supplementary information:</strong>
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<strong>Table S1</strong>: Unprocessed list of the genes in the top 25th percentile of strength of interaction with the indicated query strains, and corresponding NGR values. Note that NGR value of a target gene represents the effect of that gene deletion on growth at 19°C relative to 30°C in the query mutant background (see methods). Sheet 1 shows a list of positive genetic interactors with <em>taz1D</em>, while Sheets 2-5 show negative genetic interactors with the indicated query mutant.
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<strong>Table S2: </strong>List of genes that show negative genetic interaction specifically with <em>taz1D</em> at 30°C. The first sheet shows gene information along with respective NG and Log2 NG values (see Methods). Sheet 2 shows the GO tags for the genes from Sheet 1. Sheet 3 is a summary of Sheet 2, indicating the number of genes corresponding to each GO tag. In addition, Sheet 4 in Table S2 provides a description of the GO analysis column headings of the table represented in Sheet 2.
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<strong>Table S3:</strong> List of top 25th percentile suppressors of <em>taz1D</em> c/s<em>.</em> The sheet format follows that of Table S2, except that NGR and Log2NGR are presented (see Methods). The high confidence hits determined by the 2-standard deviation method are highlighted in purple in Sheet 1. GO tags and GO analysis summaries for the high confidence hits are presented in Sheets 2 and 3, respectively.
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<strong>Table S4:</strong> List of genes whose deletion reverts the ability of <em>rif1+</em> deletion to suppress <em>taz1D</em> c/s. The sheet format follows that of Table 2, with NGR values. These genes were identified as explained in Figure 3a and b.