Expression data from primary keratinocytes obtained from WT and K14-Cre Glut1 KO mice [MoGene-2_0]. Mus musculus

Glut1 is highly expressed in basal cells of keratinocytes, but the functions and regulation of Glut1 has not been explored, here we specifically ablate Glut1 in epidermal keratinocytes to elucidate the role of glucose transport in the skin. We performed microarray analysis in WT and Glut1 deficient primary keratinocytes to determine the pathways might contribute to the impaired proliferation in Glut1 deficient keratinocytes, Overall design: We harvested primary keratinocytes from 1 week old Glut1_fl/fl (Glut1 WT) (n=4) and Glut1_fl/fl K14 Cre (Glut-/-) (n=4) mice. Keratinocytes from each mice were seeded on one 10 cm dishes in complete KSFM medium. After 3 days, the cells were trypsinzed and reseed in new dishes with same cell number. The cells were harvested one day after harvesting. Two plates of cells of the same genotype were randomly harvested together, thus each genotype represents a combination of 2 biological replicates for microarray analysis.