Rescue of lethality of aux mutants by overexpression of epsin and/or clathrin heavy chain.

aThe flies of the genotypic classes listed were obtained from crosses of three w; gChc+/+; auxK47/TM6B males with eight w; glqf+/CyO; auxD128/TM6B virgin females, kept at 25°C, and transferred to new food vials every 2–3 days for 5 days. Flies with glqf+ only were differentiated from gChc+/glqf+ flies by the latter having darker eye color.baux means either auxK47 or auxD128cThe important comparison is between the first row and the three rows beneath. Addition of either or both Pglqf+ or PgChc+ transgenes increases drastically the viability of auxK47/auxD128 adults. It is not clear why the effect of both transgenes is not greater than the effect of a single transgene. One possibility, suggested by the expected frequency of adults (see d below) is that each transgene rescues viability completely. In this case, the differences from expectation would be due to the effects of other aspects of the genotype, such as the presence or absence of CyO, and transgene insertion sites.dThe expected numbers were calculated making three simplifying assumptions: (1) auxK47/auxD128 is completely lethal; (2) one copy of either transgene rescues viability fully; (3) no aspect of the genotype other than auxK47/auxD128 affects viability.