RDR6 gene-knockout Nicotiana benthamiana plants expressing the inverted repeat sequence of TAS3

To restore fertility with the loss of rdr6 function, the transgenic rdr6 plants which express double-stranded RNA derived from Nicotiana benthamiana TAS3 were produced (TAS3i plants) to complement the function of RDR6 in the miR390-TAS3-ARF pathway. The phenotypes of the TAS3i plants were restored to those of wild-type plants including fertility.