Expression data from adult wild-type and transgenic zebrafish revealed Oligo-Fucoidan Prevents Radiation induced fibrosis and secondary tumors in zebrafish

Radiotherapy (RT) often causes unwanted side effects such as radiation-induced fibrosis (RIF) and second malignancies (SM). Fucoidan, a sulfated polysaccharide extracted from brown seaweed, has many biological effects including anti-inflammation and anti-tumor. In the present study, we investigated the radioprotective effect of Oligo-Fucoidan (OF) using zebrafish animal model. Using an oligo-fucoidan oral gavage in wild-type and adult transgenic zebrafish, we then used microarrays to detail the global programme of gene expression after fucoidan treatment and identified distinct classes of up- and down-regulated genes during this process. Forty wild-type (WT) 5-month-old adult fish were divided into two group treated with γ-irradiation (R) and one group were fed with oligo-fucoidan for one month followed by 40 Gy γ-irradiation exposure (OF+R), two batches of WT fish were performed, the first is treated with 40 Gy and the second is treated with 10Gy. Three-month-old female HBx,Src and HBx,Src,p53–/+ transgenic zebrafish were diet-induced obesity (DIO) for two months by approximately 6.9 mg and 83 mg cysts/fish/day respectively, and then 5-month-old adult fish were divided into two group treated with γ-irradiation (R), and one group of fish oral feding with oligo-fucoidan for one month followed by γ-irradiation exposure (OF+R). The γ-irradiation exposure for HBx,Src and HBx,Src,p53–/+ were 40Gy and 10Gy respectively. OF (300 mg/kg) was administrated by oral gavage for one month starting at 4 months of age for one month. The fish were sacrificed and the hepatic tissue was immediately frozen in liquid nitrogen and stored at −80 °C for later RNA extraction and hybridization on Affymetrix microarrays.