Validation of microarray- and ChIP-chip-derived results by real-time PCR.

Total RNA used for microarray analyses was subjected to real-time PCR quantification. The standardised expression ratio (SER) indicates the expression normalised to 18S rRNA and standardised to control condition (scrambled siRNA or vehicle). A SER of 100% indicates no expression difference versus control. P-values were based on a two-tailed, unpaired t-test. SEM: standard error of the mean with respect to the intervention; n: number of single PCR measurements. Fold changes are given in percent relative to the control condition based on the microarray data. Validated Genes, i.e., those which showed significantly (p<0.05) altered mRNA levels as detected by real-time PCR and which additionally are concordantly regulated in microarray-analyses, are highlighted in bold.