Gene expression in D4 WT and P38a KO uteri

Differential expression genes were normalized to fragments per kilobase of exon model per million mapped reads (RPKM) using EdgeR package in R with the criteria of fold change significantly greater than 2 or less than 0.5 and P<0.05. Overall design: Total RNAs from D4 uteri were extracted by TRIzol and purified by ploy-A before subjected to RNA-Seq. The expression of level of each gene is normalized to RPKM.