STED image quality comparison images

fixed HeLa cell with tubulin cytoskeleton stained with Star635. The sample was imaged with Leica TCS SP5 STED, 100x/1.4 oil immersion objective (HCX PL APO CS), pinhole 1.0 AU, excitation wavelength 635 nm. The depletion laser wavelength was set to 765 nm and its intensity was varied between 50-100\% -- the same field of view was imaged sequentially with two different detectors. The image size is 700x700 and pixel size 15x15 nm.

本数据集的样本为微管细胞骨架经Star635染料染色的固定海拉（HeLa）细胞。该样本采用徕卡TCS SP5受激发射损耗（STED）显微镜进行成像，搭配HCX PL APO CS型100倍/1.4数值孔径油浸物镜，针孔孔径设置为1.0光学单位（AU），激发波长为635纳米。实验所用耗尽激光波长设定为765纳米，强度在50%至100%范围内调节；同一视场通过两台不同探测器依次完成成像。图像尺寸为700×700像素，像素尺寸为15×15纳米。

搜集汇总

背景与挑战

背景概述

该数据集包含12个STED显微镜图像，用于比较两种探测器在不同激光强度下的成像质量，样本为染色HeLa细胞微管骨架，具有详细的显微镜技术参数。数据集适用于图像处理和分析研究。