Labeled 17 Hardwood Species and 55 Genotypes of Populus Stomatal Images Datasets

Research has indicated the potential of using machine learning algorithms to detect and measure stomata automatically. However, the current limitation for further improving and fine-tuning machine learning-based stomatal study methods is due to the small, inconsistent, and monotypic nature of stomatal datasets, which are also not easily accessible. To address this issue, our collection comprises more than 11,000 unique images of hardwood leaf stomata gathered from projects conducted between 2015 and 2020-2022. The dataset includes over 7,000 images of 17 frequently encountered hardwood species, including oak, maple, ash, elm, and hickory, as well as over 3,000 images of 55 genotypes from seven Populus taxa (as detailed in Table 1). Each image has been labeled as either stomata (stomatal aperture only) or whole_stomata (stomatal aperture and guard cells) and has a corresponding YOLO label file that can be transformed to other annotation formats. These images and labels are publicly available, making it easier to train machine-learning models and examine leaf stomatal traits. By utilizing our dataset, users can (1) use state-of-the-art machine learning models to identify, count, and quantify leaf stomata; (2) investigate the diverse range of stomatal characteristics across different types of hardwood trees; and (3) create new indices for measuring stomata.