Ribosome Profiling and Single-Cell RNA Sequencing Reveal the Unfolded Protein Response as a Central Regulator of Pigeon Lactation

This study examines crop sacs from 225-day-old unpaired, non-lactating male pigeons (MN) alongside males that initiated lactation on the first day after incubation (ML). Through RNA sequencing, ribosome profiling, and single-cell transcriptome sequencing (scRNA-seq), we observed a significant upregulation of genes linked to ribosome assembly and protein synthesis in ML samples compared to MN.