Isoenzyme equipment of lactate dehydrogenase (LDH), glutamate oxaloacetate transaminase (GOT) and malate dehydrogenase (MDH) of pyruvate-low PC-3 and DU145 cells as measured by free flow isoelectric focusing.

The percentual amounts were calculated as area under the curve (AUC) in percent of the respective total enzyme activity loaded onto the focusing column. Cyto = cytosolic isoenzyme, mito = mitochondrial isoenzyme, prec = cytosolic precursor of the mitochondrial isoenzyme. Unpaired Student’s t-test was performed to assess significancy. Mean ± SEM. n = 3. P-values (PC-3 vs DU145): LDH H-type and M-type (****) < 0.0001, GOT cyto and mito (**) = 0.0035. In PC-3 cells neither supplementation of extracellular pyruvate nor RH02211 in concentration of 16 µM had an effect on the isoenzymes of LDH, GOT and MDH.