Comparative transcriptome profiling of human and pig intestinal epithelial cells after porcine deltacoronavirus infection

Porcine deltacoronavirus (PDCoV) is an emerging infectious disease of swine with zoonotic potential. Phylogenetic analysis suggests that PDCoV originated recently from a host-switching event between birds and mammals. Little is known about how PDCoV interacts with its differing hosts. Human derived cell lines are susceptible to PDCoV infection. Here we compare gene expression profiles of an established host swine cells to a potential emerging host human cells after infection with PDCoV. Cell lines derived from intestinal lineages were used to reproduce the primary sites of viral infection in the host. Porcine intestinal epithelial cells (IPEC-J2) and human intestinal epithelial cells (HIEC) were infected with PDCoV. RNA-sequencing was performed on total RNA extracted from infected cells. Our findings provide an important foundation that contributes to an understanding of the mechanisms of PDCoV infection across different hosts. To our knowledge, this is the first report of transcriptome analysis of human cells infected by PDCoV.

猪德尔塔冠状病毒（PDCoV）是一种新出现的猪只传染性疾病，具有人畜共患病潜力。系统发育分析表明，PDCoV近期起源于鸟类与哺乳类之间的宿主转换事件。关于PDCoV如何与其不同的宿主相互作用，目前所知甚少。源自人类细胞的细胞系对PDCoV感染具有易感性。本研究中，我们比较了经PDCoV感染后，已确立宿主猪细胞与潜在新兴宿主人类细胞的基因表达谱。来自肠道谱系的细胞系被用于复制宿主病毒感染的原发部位。猪肠道上皮细胞（IPEC-J2）和人肠道上皮细胞（HIEC）被感染PDCoV。对从感染细胞中提取的总RNA进行了RNA测序。我们的研究结果为理解PDCoV在不同宿主中的感染机制提供了重要的基础。据我们所知，这是首次关于PDCoV感染人类细胞转录组分析的报道。