CompareRhythms data in healthy and asthmatic airway epithelial cells

Rationale: Cellular circadian rhythms regulate multiple cellular processes and have been hypothesized to influence epithelial function in asthma. Airway resistance has circadian variability in patients with asthma, profiling of circadian rhythms of gene expression in human airway epithelia has not been performed. Organotypic cultures of primary human airway epithelial cells differentiated at an air-liquid interface recapitulate the major cell types of human airway epithelia to allow for evaluation of gene expression not possible in vivo and demonstrate rhythmicity of core circadian genes after temperature synchronization.
Methods:  Primary human airway epithelial cells from 6 healthy children and 6 children with asthma were differentiated an air-liquid interface and circadian rhythms were synchronized using cycled incubator temperature. RNA was harvested every 4 hours and RNA-sequencing performed to measure transcriptome-wide expression, with differential rhythmicity of genes identified..., AECs from healthy children and children with physician-diagnosed allergic asthma were obtained from subjects (ages 6-16) while under general anesthesia for elective procedures. AECs were expanded and at passage 3 were differentiated at an ALI in PneumaCult ALI media (Stemcell) at 37°C, producing an organotypic differentiated epithelial culture with mucociliary morphology. AECs from children were obtained under study #12490 and #1596 approved by the Seattle Children’s Hospital Institutional Review Board with investigations conducted following the rules of the Declaration of Helsinki of 1975. Temperature cycled synchronization occurred for 6 days (12 hours at 37°C, 12 hours at 34°C), then cultures were maintained at a constant 37°C for 48 hours during RNA isolation. RNA was collected every 4 hours for 48 hours, beginning 4 hours after final media change and completion of temperature synchronization. Two independent cultures were harvested for RNA at each timepoint. Samples with RIN >8 ..., , # CompareRhythms Data in healthy and asthmatic airway epithelial cells

[https://doi.org/10.5061/dryad.1jwstqk2g](https://doi.org/10.5061/dryad.1jwstqk2g)

This dataset contains the CompareRhythms input and output data on transcriptomic analyses performed in cultured primary human airway epithelial cells following temperature synchronization.

## Description of the data and file structure

AECs from healthy children and children with physician-diagnosed allergic asthma were obtained from subjects (ages 6-16) while under general anesthesia for elective procedures. AECs were expanded and at passage 3 were differentiated at an ALI in PneumaCult ALI media (Stemcell) at 37°C, producing an organotypic differentiated epithelial culture with mucociliary morphology. AECs from children were obtained under study #12490 and #1596 approved by the Seattle Children’s Hospital Institutional Review Board with investigations conducted following the rules of the Declaration of Helsinki of 1975. Temperatur...