Expression data from distinct populations of iMC from the small intestine of Ackr4(+/gfp) mice

Mesenchymal cells play key roles in modulating tissue homeostasis in various organs, including the intestine. Here we show that the murine intestinal mesenchymal cells are heterogenous, comprising at least three distinct populations. In this dataset, we include the expression data obtained from sorted subsets of CD45-Ter119-EPCAM-GP38+CD31- murine intestinal mesenchymal cell (iMC) populations. The iMC populations were separated into: CD146+ cells, which expressed CD9 and CD29, but not Ackr4, SCA1, ICAM1 or PDGFRa; GFP+ fibroblasts, which expressed Ackr4, SCA1, ICAM1 and PDGFRa; and GFP- fibroblasts, which also expressed SCA1, ICAM1 and PDGFRa, but were negative for Ackr4 expression. 8 Total samples were analyzed using Affymetrix Mouse Transcriptome arrays. 6 samples were used to generate a pairwise comparison between GFP+ vs GFP- fibroblasts. Analysis was performed using GeneSpring. Genes with an FDR≤10% and a fold-change ≥2 were selected. We did not generate pairwise comparisons for CD146+ cells vs GFP+ or GFP- fibroblasts because only two arrays were performed for CD146+ cells and data from these arrays contained significant variability.