RUVBL2 transcriptionally regulates the mitophagy pathway in gallbladder cancer cells

Gemcitabine resistance in gallbladder cancer poses a significant challenge to patient prognosis. This study identified RUVBL2 as a critical factor contributing to gemcitabine resistance in gallbladder cancer. Further analysis, combining ChIP-seq and gene enrichment studies, revealed that RUVBL2 regulates the mitophagy pathway. This regulation helps maintain intracellular redox homeostasis in gallbladder cancer cells following gemcitabine treatment. Overall design: In the human gallbladder cancer cell line NOZ (with two biological duplicates), ChIP assay was performed using an antibody against RUVBL2, followed by high-throughput sequencing.